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To the statistical analysis of bacterial abundances, relative or complete, the distribution of the info need to be deemed. Complete abundances tend to be greatly correct-skewed in distribution, so log-transformation will probably be valuable if statistical methods that assume typical distribution are used. Because the abundances are primarily bacterial counts, it's a good idea to make use of statistical checks suitable for count knowledge, which include generalized linear styles with Poisson or adverse binomial distribution.
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The sequencing reads assigned to distinct taxa in Every single sample had been divided by the full number of reads to the sample to acquire relative abundances in the taxa in Every sample. The relative abundances received determined by the sequencing reads were translated into complete abundances by multiplying the relative abundance of every taxon by the whole bacterial abundance in the sample.
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